ISOLATION OF CANCER CELL LINE FROM WOMAN BREAST TUMOR
Pham Van Phuc*, Le Thanh Trung, Truong Hai Nhung, Vuong Gia Tue, Duong Thanh Thuy, Phan Kim Ngoc
University of Science, National University Hochiminh city
Cancer cell lines have had important roles in anti-cancer drug screening research and development. Breast cancer cell lines contributed much in development of anti-cancer drugs. So that, many breast cancer cell lines have been established up to date. However, there is not breast cancer cell line from Vietnamese woman established today. The aim of this research was establishing the procedure to isolate breast cancer cell line from Vietnamese woman breast cancer tumor. In this research, breast tumors in Phase IIIA were used to isolate candidate breast cancer cells by primary culture with specific medium. The cells isolated from primary culture were subcultured some times to propagate and enrich breast cancer cells. Cancer cell population was purified by sorting breast cancer cells based on CD90 negative-expression by flow cytometry. To confirm the tumor-creating capacity, purified breast cancer cell population was checked the expression of BRCA1 gene, CD24 marker and the potency creating tumor in vivo mice. The results showed that breast cancer cell lines had homogenous phormology with epithelial shape, expressed lowly BRCA1 gene and caused the tumor in mouse when injected more than 2 million cells.
Keywords: Breast tumor, breast cancer cell line, CD24 marker, cancer cell
Fulltext: Journal of Biotechnology 8(4): 1775-1783, 2010