Transplantation of non-expanded adipose stromal vascular fraction and platelet rich plasma for articular cartilage injury treatment in mice model
Phuc Pham Van, Khanh Bui-Hong-Thien, Dat Ngo-Quoc, Lam Khuat-Tan, and Ngoc Phan-Kim
1Laboratory of Stem Cell Research and Application, University of Science, Vietnam National University, Ho Chi Minh city, Vietnam
2University of Medical Center, Ho Chi Minh University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam
3Department of Pathology, University ofMedicine and Pharmacy, Ho Chi Minh City, Vietnam Corresponding author: pvphuc@hcmuns.edu.vn
Received 13 August 2012; Accepted 25 December 2012
Abstract
Stromal vascular fraction (SVF) combined with platelet rich plasma (PRP) commonly used in preclinical and clinical osteoarthritis as well as articular cartilage injury treatment. However, this therapy has not been carefully evaluated the safety and efficacy. This research aims to assess the safety and efficacy of SVF combined with PRP transplantation. Ten samples of SVFs and PRPs from donors were used in this research. About safety, we evaluate the expression of some genes related to tumor formation such as Oct-4, Nanog, SSEA3, SSEA4 by RT-PCR, flow cytometry and tumor formation when injected in NOD/SCID mice. About efficacy, SVF was injected with PRP into murine joint that caused joint failure. The results showed that SVFs are negative with Oct-4, Nanog, SSEA-3, SSEA-4 as well as can not cause tumors in mice. SVFs combined with PRP can improve the joint regeneration in mice. These results proved that SVFs combined with PRP transplantation is a promising therapy for articular cartilage injury treatment.
Keywords: Adipose stromal vascular fraction, joint failure, osteoarthritis, platelet rich plasma, mesenchymal stem cells.
