CN. Nguyễn Gia Khuê

Master of Science Candidate

Animal Physiology

University of Science, Vietnam National University, Ho chi Minh City, Vietnam (2011-2013)

Bachelor of Science

Biology

University of Science, Vietnam National University, Ho chi Minh City, Vietnam (2006-2010)

2013 – now: Research Assistant at Laboratory of Vaccine and Immunotherapy Delivery. University of Arkansas, Fayetteville.

2010 – 2013: Researcher – Laboratory of Stem cell Research and Application; Teaching Assistant – Faculty of Biology, University of Science, Vietnam National University, Ho Chi Minh City, Vietnam.

Research projects:

• Research on establishing Vietnamese cancer stem cell line expressing GFP
• Isolation, culture and cryopreservation of human bone marrow – derived mesenchymal stem cells
• Study on treatment of diabetes – type 1 by stem cell therapy

Teaching Assistant for classes:

• Labwork on Animal Physiology
• Labwork on Pathology and Immunity
• Stem cell Technology
• Animal cell culture Technique

2006 – 2010: Undergraduate student, University of Science, Vietnam National University, Ho Chi Minh City, Vietnam. GPA: 3.37/4.00

Undergraduate thesis:

• Research on differentiating mesenchymal stem cell to insulin producing cell and estimate their homing ability in mouse models of type I diabetes mellitus

Research skills:

• Molecular manipulation: Plasmid extraction, DNA – RNA extraction, PCR, RT-PCR, qPCR, Electrophoresis
• Cell culture technique: Trypsinization, Cryoprotection, Cell thawing, Isolation cell from blood or tissue.
• Transgenic technique: Calcium phosphate method, Liposome method (Lipofectamine, Fugene), Virus method (Retrovirus vector, Lentivirus vector)
• Bioinformatics: Primer designing, Data analysis by Excel software (Microsoft Office)

Papers

3. Phuc Van Pham, Phuoc Thi-My Nguyen, Anh Thai-Quynh Nguyen, Vuong Minh Pham, Anh Nguyen-Tu Bui, Loan Thi-Tung Dang, Khue Gia Nguyen, Ngoc Kim Phan. Improved differentiation of umbilical cord blood-derived mesenchymal stem cells into insulin-producing cells by PDX-1 mRNA transfection. Differentiation. 2014:87 (5) 200-208.

2. Phuc V. Pham, Ngoc B. Vu, Thuy T. Duong, Tam T. Nguyen, Nhung H. Truong, Nhan L.C. Phan, Tue G. Vuong, Viet Q. Pham, Hoang M. Nguyen, Kha T. Nguyen, Nhung T. Nguyen, Khue G. Nguyen, Lam T. Khat, Dong V. Le, Kiet D. Truong, Ngoc K. Phan. Suppression of human breast tumors in NOD/SCID mice by CD44 shRNA gene therapy combined with doxorubicin treatment. OncoTargets and Therapy. 2012:5 77–84.

1. Doan Chinh Chung, Vu Bich Ngoc, Nguyen Gia Khue, Do Minh Si, Phan Kim Ngoc, Pham Van Phuc. Isolation and cryopreservation of human bone marrow derived mesenchymal stem cells. International Journal of Plant, Animal and Environmental Sciences. 2012:2 83-90

Conference reports

4. Khue G. Nguyen, Bhanu Koppolu, Sean G. Smith, Sruthi Ravindranathan, David A. Zaharoff. Interleukin-12 elicits various responses of splenocytes from different mouse strains. Immunology 2015 – AAI Annual Meeting.

3. Sean Smith, Sruthi Ravindranathan, Khue Nguyen, David Zaharoff. Mathematical model of protein delivery within the Urinary Bladder. Biomedical Engineering Society (BMES), 2014.

2. Doan Chinh Chung, Vu Bich Ngoc, Nguyen Gia Khue, Do Minh Si, Phan Kim Ngoc, Pham Van Phuc. Isolation and cryopreservation of human bone marrow derived mesenchymal stem cells. Reported at Fourth International Conference on Biomedical Engineering in Vietnam. 2012; The Asian Federation of Biotechnology – Regional Symposium in Vietnam, 2012.

1. Nguyen Gia Khue, Pham Minh Vuong, Doan Ngoc Trung, Nguyen Vuong Tuong Vy, Le Thi Bich Phuong, Phan Lu Chinh Nhan, Pham Van Phuc, Phan Kim Ngoc. Differentiating adipose derived stem cells into insulin producing cells and evaluating insulin secretion of insulin producing cells. 8^th Annual Scientific Conference, University of Science, Vietnam National University, 2012.

• The excellent presentation award of presentation contest of the Laboratory of Stem cell Research and Application, 2011, 2012
• The 1^st prize of the annual contests of “Mendel Arboretum”, University of Science, 2010
• “5 good aspects” Student, Vietnam National University – Ho Chi Minh City, 2010
• “3 good aspects” Student, University of Science, 2007
• University of Science Scholarship for 5 out of 8 semesters, 2006-2010

Name:

Date of Birth:

Place of Birth:

Nationality:

Se
x:

Current address:

Phone (Lab):

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Khue Gia Nguyen

Nov. 13, 1988

Soc Trang City, Vietnam

Vietnamese

Male

Laboratory of Stem Cell Research and Application, University of Science, Vietnam National University of Ho Chi Minh City, 227 Nguyen Van Cu Street, Dist 5, Ho Chi Minh City, Vietnam

84-8-38-397-719

84-8-38-350-096

84-91-6371-227

ngkhue@hcmus.edu.vn; gkst1988@gmail.com